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Monday, April 13, 2020 | History

2 edition of Immobilised lipase biocatalyst in solvent-free medium found in the catalog.

Immobilised lipase biocatalyst in solvent-free medium

Yee Peng Yong

Immobilised lipase biocatalyst in solvent-free medium

immobilisation studies and enzymatic kinetics

by Yee Peng Yong

  • 159 Want to read
  • 12 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D) - University of Birmingham, School of Chemical Engineering, Faculty of Engineering, 1999.

Statementby Yee Peng Yong.
ID Numbers
Open LibraryOL18389113M

Even it is possible to use this supported biocatalyst for hydrolytic processes (e.g. Refs. [, , , , ]), if it is provided the use of a reaction media consisting in a water-saturated organic solvent; in these cases, the low water content of the medium will not contribute to desorb the lipase from the support, and the biocatalyst. Biblioteca en línea. Materiales de aprendizaje gratuitos. Denise Maria Guimarães Freire - A pesquisadora é coordenadora do Laboratório de Biotecnologia Microbiana (LaBiM) sendo atualmente Pró-reitora de Pós-graduação e Pesquisa da Universidade Federal do Rio de Janeiro e professor Titular do Instituto de Química Depto. de Bioquímica da Universidade Federal do Rio de Janeiro. Coordenou o Programa de Pós .


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Immobilised lipase biocatalyst in solvent-free medium by Yee Peng Yong Download PDF EPUB FB2

Lipase catalyzed esterification of palmitic acid with ethanol in the presence of Lipozyme IM 20 in a solvent free medium was investigated by Vieira et al. Different acid/alcohol molar ratios were tried as, and Cited by: Optimized conditions for the transesterification of Castor oil with propanol as acyl acceptor in solvent free medium were, 10% immobilized lipase relying on oil-weight, molar ratio of alcohol.

Esterification of polyglycerol with polycondensed ricinoleic acid catalysed Immobilised lipase biocatalyst in solvent-free medium book immobilised Rhizopus oryzae lipase Rhizopus oryzae lipase in a solvent-free medium. The great advantage of using.

Alkyl esters are high added value products useful in a wide range of industrial sectors. A methodology based on a simple sol-gel approach (biosilicification) is herein proposed to encapsulate enzymes in order to design highly active and stable biocatalysts.

Their performance was assessed through the optimization of valeric acid esterification evaluating the effect of Cited by: 4.

Immobilised enzymes, the pros and cons of their use in large industrial processes are reviewed. Parameters for economically viable biocatalytic processes: recyclability, enzyme selectivity and reaction turnover are discussed. Large-scale applications of immobilized enzymes in the food, chemical, pharmaceutical, cosmetic and medical device Cited by: 7.

Immobilization of lipase. The first immobilized enzyme was reported by Nilson and Grifflin more than a century ago, while Chibata and co-workers developed the first industrial immobilized enzyme, i.e., Aspergillus oryzae aminoacylase for synthetizing racemic D – L amino recent years, production of biodiesel using immobilized lipases has attracted great by: Lipase from Pseudomonas fluorescens Immobilised lipase biocatalyst in solvent-free medium book on silica modified with octyl groups (PFL-octyl-silica biocatalyst) presented similar performance and operational stability compared to the covalent commercial preparation of the same lipase or other lipases in the ester syntheses with flavor properties.

On the other hand, different interactions Cited by: 4. Biocatalysis in Supercritical Fluids, in Fluorous Solvents, and under Solvent-Free Conditions Helen R. Hobbs, and Neil R. Thomas * School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, United KingdomCited by:   The hyper-activated biocatalyst was more efficient than the previously reported silica-bound lipase with minimum leaching of the enzyme from the reaction mixture.

The K m and V max of the free ( mM and μmol min−1 mL−1, respectively) and silica-bound lipase ( mM and μmol min−1 mg−1, respectively) were determined Cited by:   This work illustrates the enzymatic synthesis of cinnamyl butyrate by esterification of butyric acid and cinnamyl alcohol.

Experiments were performed to study the various operating parameters such as molar ratio, enzyme concentration, temperature, and speed of agitation. Also, the suitable kinetic model for esterification reaction was predicted and the various kinetic Cited by: 6.

The specificity of the A-lipase from Candida antarctica (CALA) has been characterized to further clarify the scope of the biocatalyst. The lipase was found to exhibit an almost uniform activity towards various straight-chained primary alcohols and carboxylic acids, only exhibiting a low activity towards very short-chained acids.

More interestingly, the enzyme was found to exhibit Cited by: Abstract. Among the hydrolytic enzymes, transformations mediated by lipases have been extensively studied.

This chapter begins with a brief description on the three lipases – porcine pancreas lipase, Rhizomucor lipase and Candida rugosa lipase – employed extensively in the transformation work described in this book. This is followed by specificity of lipases and the.

Enter search terms. Keep search filters New search. Advanced search. Abstract Enzymatic kinetic resolution reactions are a well-established way to achieve optically active compounds.

When enzymatic reactions are combined to continuous-flow methodologies, other benefits are added, including reproducibility, optimized energy use, minimized waste generation, among others. In this context, we herein report a case study Cited by: 6.

Lipase-catalyzed resolution of 1,2-diols p. Partitioning of water during the production of terpene esters using immobilized lipase p. Thermoinactivation of polyphenol oxidase in organic solvents with low water content p.

Continuous enzymatic transesterification of rapeseed oil and lauric acid in a solvent-free system p. Dicaprin was obtained from glycerol and ethyl caprate in a solvent-free system in yields up to 88% (Rhizopus arrhizus, 15±50 °C) Edmundo et al 75 prepared mono-olein (71% conversion, % pure after passage through a silica column) from glycerol on silica, oleic acid, and Mucor miehei lipase in 2-methyl2-butanol/hexane () at 40 °C.

REVIEW ♦ Asalapuram R. Pavankumar and Krishnan Sankaran The Need and New Tools for Surveillance of Escherichia coli Pathogens Food Technology and Biotechnology 46 (2) – ()» Abstract |» Full Text (PDF) |» Abstract (Croatian). The food additive named polyglycerol polyricinoleate (PGPR) and identified with the code E (PGPR) is used as emulsifier in tin-greasing emulsions for the baking trade and for the production of low-fat spreads.

However, the main application of PGPR is in the chocolate industry, where, besides its action as an emulsifier, it also has important properties as a viscosity modifier and. Lipase-catalyzed interesterification of egg-yolk phosphatidylcholine and plant oils / A.

Chojnacka, W. Gładkowski, G. Kiełbowicz, A. Gliszczyńska, N. Niezgoda, et al. Plant Products for Pharmacology: Application of Enzymes in Their TransformationsCited by:   The potential of lipases (E.C) as biocatalysts for the production of fatty acid derivatives for the food and nutraceutical industries, such as flavouring esters, fatty acid esters of antioxidants and structured lipids, is enormous, mainly due to their high regio- and stereo-selectivities, in addition to the other well-known advantages of enzymatic processes.

The book draws on the expert knowledge of contributors with backgrounds both in industry and academia. The following summary will guide you through the book: The book starts with a short overview of the industry, including historical and economic aspects as well as current trends and future perspectives.

Biodiesel Education Program at the University of Idaho. If you are looking for any biodiesel related information, this website provides you with an unbiased information through peer-reviewed publications, videos, technical notes, and thousands to literature organized with the topics you are looking for.

This should be the first (and probably the last) page you will need to get information. A previous study focused on the esterification reaction of fusel oil and oleic acid, in which immobilised Novozym ® lipase enzyme was used as a biocatalyst.

Compared to the product obtained by acid catalysis, in the esterification reaction, there was no trace of oleic acid since the entire conversion was achieved by continuous water Cited by: Lipase B from C. antarctica (CALB) is the most widely employed biocatalyst in these reactions, mainly due to its selectivity and high acylation rates.

Several factors, such as the composition and water content of the reaction medium, the nature and chain length of the acyl donor, the acyl donor/flavonoid molar ratio, and temperature, may affect Cited by: In situ ATR‐FTIR spectroscopy has been successfully used to monitor the concentrations of mono‐, di‐ and triglycerides in the solvent‐free esterification of lauric acid and glycerol (Mueller et al., ).

The full hydrolysis of castor oil has been monitored with ATR‐FTIR spectroscopy (Khaskheli et al., ). Yet, in this application. Comparing these results with those obtained with the soluble enzymes, it can be observed that the acid values reached with the immobilized derivatives ( mg KOH/g with lipase from Rhizopus arrhizus and mg KOH/g with lipase from Rhizopus oryzae) were similar to those obtained with the soluble lipases ( mg KOH/g and mg KOH/g.

A previous study focused on the esterification reaction of fusel oil and oleic acid, in which immobilised Novozym ® lipase enzyme was used as a biocatalyst.

Compared to the product obtained by acid catalysis, in the esterification reaction, there was no trace of oleic acid since the entire conversion was achieved by continuous water. John CV 1. “Enzymatic synthesis of phenolic lipids in solvent-free medium using flaxseed oil and 3,4-dihydroxyphenyl acetic acid”.

“Esterification of benzoic acid and glycerol to 1-monobenzoate glycerol in solventless media using an industrial free Candida antarctica lipase B”.“Lipase catalyzed synthesis of. Industrial Biorefineries and White Biotechnology –Internal structures of a corn kernel.

PERICARP STARCH FLOURY ENDOSPERM (%) HULL AND FIBER (%) STARCH AND GLUTEN VITREOUS GERM (%) TIP CAP (%) –Proximate chemical analysis of corn grain Compound Average % Mass (dry basis) Starch Hemicellulose Cellulose + Lignin Protein Fat Ash.

Full text of "Thermodynamics in Biochemical Engineering" See other formats. موارد یافت شده: 1 - Optimization of Quadrilateral Infiltration Trench Using Numerical Modeling and Taguchi Approach (چکیده) 2 - Reconstruction of Caspian Sea level changes using magnetic susceptibility during the last millennium (چکیده) 3 - Optimization of fuel ratio in solution combustion method for fabrication of nickel aluminate spinel used in the esterification.

A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an in. Often, there is no alternative route for their synthesis but fermentation. Also bioconversion reactions, based on the use of (immobilised) microbial biocatalysts (cells or enzymes), yield useful interesting regio- and enantioselective molecules under mild reaction conditions, often starting from racemic precursors (Vandamme et al.).

(چکیده) 19 - The impact of US monetary policy uncertainties on oil and gas return volatility in the futures and spot markets (چکیده) 20 - A Randomized Clinical Trial of Laser Hemorrhoidoplasty vs Milligan and Morgan Hemorrhoidectomy (چکیده) 21 - An agent-based coupled heat and water transfer model for air frying of doughnut as a.

The book will provide valuable reference and learning material to other researchers and students in the field, with the references at the end of each chapter serving as guidance to further reading material. My sincere gratitude is expressed to the contributing authors.

O Scribd é o maior site social de leitura e publicação do mundo. Lipase produced by Rhizomucor miehei enhanced the level of Larios et al. [] have shown that C. antarctica lipase frac- polyunsaturated fatty acid observed by reduction in total lipid tion B (CAL-B) can be employed as a robust biocatalyst in content [].

esterification reactions due to the high conversions obtained in the synthesis of short. The enzyme biocatalyst is simply an acetone precipitated enzyme powder or a lyophilized preparation that is suspended in the organic medium in which it is completely insoluble (Zaks and Klibanov a); immobilization is then unnecessary since the enzyme is already insolubilized in the reaction medium, even though it might help by offering an.

Some works illustrate the feasibility of solvent-free sys- tems for lipase-catalysed ester synthesis reactions, and high yields have been reported (96,98,99,–). Nevertheless, mass transfer limitations and the subse - quent low reaction rates may also occur in this case, due to the characteristic heterogeneity of the reactions (li.

BIOCATALYSIS RESEARCH PROGRESS No part of this digital document may be reproduced, stored in a retrieval system or transmitted in any form or by any means. The publisher has taken reasonable care in the preparation of this digital document, but makes no expressed or implied warranty of any kind and assumes no responsibility for any errors or.

Title: Book of Abstracts of MICROBIOTEC09 Edited by The Organizing Committee of MicroBiotec09 (José A. Teixeira, Ilda Sanches, Eugénio Ferreira, Álvaro Fonseca, António Vicente, Armando Venâncio, Duarte Oliveira, Filomena Martins Pereira, Isabel Couto, Lucília Domingues) First edition November ISBN: Cover design.

The resulting strain was incubated in LB medium at 37 °C in Erlenmeyer flasks and much more 3-methylbutanol ( mg/L) than isobutanol (24 mg/L) was produced. However, in 5 g/L glucose-containing medium, the production of two alcohols was similar, and mg/L for C4 (isobutanol) and C5 (3-methylbutanol) alcohol, respectively.

Optimum temperature of amylase, lipase, protease and trypsin 50, 60 and 30 °C, respectively of both extracts. More than 80 % temperature stability of amylase, lipase, protease and trypsin were retained at mentioned optimum temperature in water soluble extracts of both treated samples.